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A Ticket Booking System For Theatre

A Ticket Booking System For Theater The motivation behind the online ticket booking framework is to give another approach to buying film ...

Tuesday, May 12, 2020

Quotes by Comedian and Actress Mae West

Mae West was best known as a comedian and actress whose bawdy plays and movies were full of double entendres and attracted the attention of industry censors. Less well known is her role as writer in many productions. Her film career—mainly at Paramount in the 1930s—seemed to stop in 1943, but she returned in the 1970s for cult classic Myra Breckinridge and Sextette. Selected Mae West Quotations †¢ I believe in censorship. I made a fortune out of it. †¢ If I asked for a cup of coffee, someone would search for the double meaning. †¢ I wrote the story myself. Its all about a girl who lost her reputation but never missed it. †¢ When Im good, Im very good. When Im bad, Im better. †¢ Too much of a good thing can be wonderful. †¢ Its hard to be funny when you have to be clean. †¢ Im a woman of very few words, but lots of action. †¢ I generally avoid temptation unless I cant resist it. †¢ It isnt what I do, but how I do it. It isnt what I say, but how I say it, and how I look when I do it and say it. †¢ Ill try anything once, twice if I like it, three times to make sure. †¢ When choosing between two evils, I always like to try the one Ive never tried before. †¢ Marriage is a great institution, but Im not ready for an institution yet. †¢ I used to be Snow White, but I drifted. †¢ Why dont you come on up and see me sometime—when Ive got nothin on but the radio. †¢ Why dont you come up sometime and see me? Im home every evening. Come up. Ill tell your fortune. Ah, you can be had. †¢ Lifes just a merry-go-round. Come on up. You might get a brass ring. †¢ Shes the kind of girl who climbed the ladder of success wrong by wrong. †¢ When women go wrong, men go right after them. †¢ There are no good girls gone wrong, just bad girls found out. †¢ Is that a banana in your pocket, or are you just happy to see me? †¢ It is better to be looked over than overlooked. †¢ Keep a diary, and someday itll keep you. †¢ To err is human, but it feels divine. †¢ Love conquers all things except poverty and toothache. †¢ Ive been rich and Ive been poor. Believe me, rich is better. †¢ Sex is emotion in motion. †¢ Virtue has its own reward, but no sale at the box office. †¢ Those who are easily shocked should be shocked more often. †¢ I like my clothes to be tight enough to show Im a woman, but loose enough to show Im a lady. †¢ Youre never too old to become younger. †¢ I like restraint, if it doesnt go too far. †¢ Im the lady who works at Paramount all day... and Fox all night. †¢ I never worry about diets. The only carrots that interest me are the number you get in a diamond. †¢ A dame that knows the ropes isnt likely to get tied up. †¢ Love thy neighbor—and if he happens to be tall, debonair, and devastating, it will be that much easier. †¢ Its all right for a perfect stranger to kiss your hand as long as hes perfect. †¢ Its not the men in my life that count, its the life in my men. †¢ Every man I meet wants to protect me. I cant figure out what from. †¢ A man in the house is worth two in the street. †¢ A girl in the convertible is worth five in the phone book. †¢ A hard man is good to find. †¢ Ten men waiting for me at the door? Send one of them home, Im tired. †¢ Give a man a free hand and hell run it all over you. †¢ A man can be short and dumpy and getting bald but if he has fire, women will like him. †¢ An ounce of performance is worth pounds of promises. †¢ Dont keep a man guessing too long—hes sure to find the answer somewhere else. †¢ Look your best—who said love is blind? †¢ Flattery will get you everywhere. †¢ Ive been things and seen places. †¢ Im no angel, but Ive spread my wings a bit. †¢ The score never interested me, only the game. †¢ Men are my hobby. If I ever got married Id have to give it up. †¢ So many men... so little time. †¢ I only like two kinds of men—domestic and imported. †¢ I only have yes men around me. Who needs no men? †¢ Save a boyfriend for a rainy day—and another, in case it doesnt rain. †¢ Some men are all right in their place—if they only knew the right places! †¢ I like a man whos good, but not too good... for the good die young, and I hate a dead one. †¢ I feel like a million tonight. But one at a time. †¢ Men are easy to get but hard to keep. †¢ Men? Sure, Ive known lots of them. But I never found one I liked well enough to marry. Besides, Ive always been busy with my work. Marriage is a career in itself and to make a success of it youve got to keep working at it. So until I can give the proper amount of time to marriage, Ill stay single. †¢ His mother should have thrown him out and kept the stork. †¢ Hes the kind of man a woman would have to marry to get rid of. †¢ Dont marry a man to reform him. Thats what reform schools are for. †¢ Opportunity knocks for every man, but you have to give a woman a ring. †¢ It takes two to get one in trouble. †¢ Too many girls follow the line of least resistance, but a good line is hard to resist. †¢ Good women are no fun. The only good woman I can recall in history was  Betsy Ross. And all she ever made was a flag. †¢ Good sex is like good Bridge. If you dont have a good partner, youd better have a good hand. †¢ Diamonds is my career. †¢ When it comes to finances, remember that there are no withholding taxes on the wages of sin. †¢ No gold-digging for me. I take diamonds! We may be off the gold standard someday. †¢ You can do what you want, but saving love doesnt bring any interest. †¢ Any time you got nothing to do and lots of time to do it, come on up. †¢ Anything worth doing is worth doing slowly. †¢ Kiss and make up—but too much makeup has ruined many a kiss. †¢ Brains are an asset to the woman in love whos smart enough to hide em. †¢ Some women pick men to marry—and others pick them to pieces. †¢ Say what you want about long dresses, but they cover a multitude of shins. †¢ Let men see whats coming to them, and women will get whats coming to them. †¢ Cultivate your curves—they may be dangerous but they wont be avoided. †¢ I didnt discover curves; I only uncovered them. †¢ The curve is more powerful than the sword. †¢ Ive been in more laps than a napkin. †¢ Id like to see Paris before I die. Philadelphia will do. †¢ I see youre a man with ideals. I better be going before youve still got them. †¢ Women with pasts interest men because men hope that history will repeat itself. †¢ Dont ever make the same mistake twice, unless it pays. †¢ Right now I think censorship is necessary; the things theyre doing and saying in films right now just shouldnt be allowed. Theres no dignity anymore and I think thats very important. †¢ The best way to behave is to misbehave. †¢ You only live once, but if you do it right, once is enough. Quote collection assembled by Jone Johnson Lewis. Each quotation page in this collection and the entire collection  © Jone Johnson Lewis. This is an informal collection assembled over many years. I regret that I am not be able to provide the original source if it is not listed with the quote.

Wednesday, May 6, 2020

Stephens vs Lincoln Free Essays

Stephens vs. Lincoln In two very different speeches by Alexander H. Stephens and Abraham Lincoln they both argue their points of view on slavery and the opposing side plus how they would like to have the new government to be run. We will write a custom essay sample on Stephens vs Lincoln or any similar topic only for you Order Now In the first speech by Stephens he states that the ideas of slavery at the time of the old constitution were wrong and that they rested on the beliefs of equality between the races. He goes on to argue that slavery in the south will have its foundations laid upon the ideas that, as Stephens says. the negro si not equal to the white man; that slavery, subornation to the superior race, is his natural and moral condition. † In contrast, Lincoln describes how each person has a different definition for the word Liberty. One side defines it as meaning that they can do what they please with themselves and the earnings. While the other defines it, as Lincoln says, â€Å"some men do as they please with other men, and the product of their men’s labor. † Lincoln identifies the central differences between the north and the south as a misconception of the word Liberty, one side called liberty and the other tyranny. Stephens believes himself, and all whites as being superior to African Americans and that it’s the law of nature for it to be so. Whereas Lincoln says that everyone is equal and that’s Stephens and others like him have destroyed the meaning and significance of the word Liberty. These opposing speeches show the points of view on slavery from the north and the south and how each side takes liberty and changes it around to the advantage of their own purposes. How to cite Stephens vs Lincoln, Essay examples

Sunday, May 3, 2020

Australian Fashion Design and Cultural Identity- MyAssignmenthelp

Question: Discuss about theAustralian Fashion Design and Cultural Identity. Answer: Introduction Fashion is shaped on the basis of national culture. However, based on the historical foundations, the Australian fashion is mainly shaped through the models from Britain, which have been evolved internationally. However, the modern Australia has a unique style of fashion, which is able to be clearly distinguished from European fashion trends. It has been revealed from the fashion brands principles in Australia; the designers are continuously trying to keep balance between creativity and national identity (Entwistle, 2015). All the designer brands attempt to integrate unique features, symbolizing the particular cultural identities. In Australian culture, fashion is distinguished from dress not by only how the fashion been fashioned or created rather by its nature. Moreover, fashion is represented by reflecting the prevailing styles in polite society instead or being based on function. Identity is one of the most compelling and contentious concepts in humanities and social science. Fas hion is significantly linked with constructing and reconstructing identity, i.e. how people represent the contradictions and self in their daily life. Through appearance and style people represents their wishes and thus it is a metaphor for identity (Bulmer Buchanan-Oliver, 2010). While considering a particular fashion cloth, i.e. skirt, Australian culture significantly represents a difference from the European culture. For instance, Australian culture believes in wearing such cloths that would help them to be relaxed. Thus, in contrast to the full or knee length skirts preferred by the European culture, the cloth has evolved to miniskirts in Australian culture. In also the indigenous culture, skirts are favoured in Australia, although the materials for those clothes are different from the non-indigenous Australian culture. Moreover, Australian culture promotes colourful representation and establishing a link with nature (Gibson, 2016). Thus the floral and fabric work is preferred in skirts. The fashion appearance in Australia has been represented the influence from 1960s fashion design and those clothes are modified and integrated in new forms in the current Australian fashion culture. For instance, during 1960s, the sarong design became very popular among th e Australian people. Additionally, during 1975, specialized hand prints in the t-shirts, sarongs and accessories have been shown to be prevalent. According to the trends and tradition of sarong skirts currently Australian labels are exploring their designs to boutiques and department stores in Europe, Asia, US and other countries throughout the world (Geczy Karaminas, 2012). Therefore, Australian brands are attempting to promote their global presence, which included Gorman brand for their unique style of skirt. Fashion weeks at Sydney and Melbourne represent different unique combination of Australian tradition with the modern clothing principles like balance between colour, texture, cutting, pattern and combination. In this assignment of Australian clothing and fashion culture analysis, the renowned fashion clothing brand has been selected, i.e. the Gorman brand, which have significantly looking for aligning their modern fashion and clothing designs with the Australian culture and evolving lifestyle. In this context, the brand especially focuses upon maintaining the quality of fabrics, shapes and customized prints, which modern Australian generation is looking for, while maintaining a link with the national culture (Healey, 2010). The founder of the brand, i.e. Lisa Gorman launcher her eponymous label at fledgling boutique in 1999 in Melbourne, with her latest collection known as less than 12 degrees, which got an instant success. The entrepreneur identified something special in her brand and followed that unique trend in her designs through years; as a result Gorman label became an iconic part of the Australian fashion industry. The reason behind its flourish is the unique thought of the owner to make a strong link between the traditional Australian culture and the specialized needs of modern young generation through the unique texture, fibre and colour concepts. Although the organization was started at Melbourne, which is limited within the home country, the online presence of the brand is making its designer clothes available to women throughout the world. Starting from a status as one-lady-with-sewing-machine-in-her-bedroom the founder has adopted unique way to turn her small business into a major operation, without slowing down (Wilcox, 2017). The brand, with other womens outfit, prioritizes skirts, which is a preferred outfit by the Australian women as casual, professional as well as occasional outfit. It is in the fashion since 1960s and the brand Garmon is attempting to keep an essence of Australian traditional symbol in the modern skirt design. For instance, The philosophy of the organization is that it consist a distinct handwriting underpinning all of the designs, including its signature prints, directional knitwear and distinctive colour palettes (Gorman Online, 2017). In addition, the brand is thoroughly modern for all of its tribal and vintage references and the inspiration from the local culture and artist surrounding the brand is significantly inspiring its designs, especially the culture of the founders local fitzroy. In addition, to symbolize the culture in the clothing, the flora and fauna, which are native to the continent, like Kangaroo, emu, wattle are used sometimes in designs. For instance, in sarongs, these symbols are commonly utilized in designs. The brand uses different types of fabrics and textures, aligning with the style and pattern suitable for the residents. The founder inspires the designers to include their cultural experience in their designs. For instance, the city, the person or a song along with a touch of m odernism. In addition, Australians are nature lover and like to be relaxed, thus the brand attempts to incorporate natural art, i.e. sunset or flower, representing each designs with colourful background, as per the likings of people (McRobbie, 2013). The organization includes silhouette prints for representing relaxed mood, floral and other colourful patters for playful representation along with a urban touch in their materials and colours. The unique pattern of the skirts also includes a beachy and natural feel. The formal collection for skirts is made on the basis of visual communication principles, like maintaining a proper balance between colours. As equal distribution of colour makes communication boring, unequal distribution of colours would be required (Garling et al., 2013). The patterns are unique for the brand that is why the brand is getting global appearance quickly. On the other hand, the casual skirts, like wrap over, miniskirts and sarong and knee length skirts include feature rope, coral, rock patterns, slit, star fish or shells and loose fabric. To represent more on culture and climate, they also launches a seasonal clothes, for instance, in summer or spring season, light pattern and fabric is used for creating more refreshing and colourful designs, according to their relaxing nature (Carrico Kim, 2014). For this, they use light soft fabric with lace and overlays, which gives an essence of feminine loo k. On the other hand, the formal skirts are more sensitively designed to eliminate any symbols of boldness. In addition to the consideration of cultural attributes, the organization is also focusing upon the social well being also. For instance, the brand introduced Gorman organic in 2007, for responding towards increasing environmental awareness. Thus, the organization started to focus more on manufacturing innovative organic fabric. In addition, the organization blended their innovation throughout their traditional cultural collection, thereby offering customers to make a sustainable choice, instead of compromising good design and quality. In this context, all the garments produced and offered by the brand are made of certified organic materials or comes from sustainable farming or non-chemical processing of via closed loop production means. In this context, to be culturally identical, the designs and patterns are made unique, which can be significantly distinguishable from the other nations Yu et al., 2014). However, the brand follows the traditional western culture through their global presence. The visual communication is aligned with the cultural traits, aligning with the design principle and values. For instance, the traditional occasional skirts are made through the use of warm and dark colours, as these colours contain more visual weight than cool and light colours. The combination of colour and patterns follows the 6 design principles and values. Longer, wider lines are included, while maintaining a visual balance in colour, texture, pattern and shape in formal and party wears, whereas for beach or summer skirts, the short and thinner lines along with soft, fine textures are used (Baird Collias, 2014). Figure: Gorman skirts (left: relaxed, middle: professional, right: occasional) (Source: Gorman Online, 2017) Analyzing the cultural attributes of Australian cloth company Gorman, it has been revealed that the brand has several strength as well as weaknesses. For instance, the organization is continuously working on improving their production, through innovation and making their products customer-centric as well as environment friendly, as a result the brand has a significant chance of global expansion. However, one of its key weaknesses is less offline presence; the brand is providing more concentration on its online presence. Thus, it can be interpreted that this area should be improved significantly. Reference List Baird, D. G., Collias, D. I. (2014).Polymer processing: principles and design. John Wiley Sons. Bulmer, S., Buchanan-Oliver, M. (2010). Experiences of brands and national identity. Australian Marketing Journal 18(4), 199-205. Carrico, M., Kim, V. (2014). Expanding zero-waste design practices: a discussion paper.International Journal of Fashion Design, Technology and Education,7(1), 58-64. Entwistle, J. (2015). The fashioned body: Fashion, dress, and social theory. John Wiley Sons. Fletcher, K. (2010). Slow fashion: An invitation for systems change. Fashion practice. The Journal of Design, Creative Process and the Fashion Industry, 259-266. Garling, S., Hunt, J., Smith, D., Sanders, W. (2013).Contested governance: culture, power and institutions in Indigenous Australia(p. 351). ANU Press. Geczy, A., Karaminas, V. (2012). Fashion and art. London: Berg. Gibson, C. (2016). How clothing design and cultural industries refashioned frontier masculinities: a historical geography of Western wear.Gender, Place Culture,23(5), 733-752. Gorman Online. (2017).Gorman Online :: About Us.Gorman Online. Retrieved 2 June 2017, from https://www.gormanshop.com.au/aboutus/ Gregory, D. (2004). It's all about Australia, mate. Auckland: Exisle Publishers. Healey, R. (2010). Global positioning of Australian fashion. New York: Berg. McRobbie, A. (2013).In the culture society: Art, fashion and popular music. Routledge. Wilcox, C. (2017). Badge, boot, button: The story of Australian uniforms. Canberra: National Library of Australia. Yu, W., Fan, J., Ng, S. P., Harlock, S. (2014).Innovation and technology of women's intimate apparel. Woodhead Publishing.

Thursday, March 26, 2020

Microsoft vs. Apple

Introduction Apple incorporation is an international company that deals in electronic gadgets, computer software and hardware. Its operations began on 1ST April 1976 through the efforts of Steve Jobs and Steve Wozniak as the founders. Apple incorporation mainly specializes in the manufacture of iPhone, iPod, Macintosh computers and iPad which is the latest computer technology. It is currently the world’s largest technology company and the second largest in the world after Exxon Mobil.Advertising We will write a custom essay sample on Microsoft vs. Apple specifically for you for only $16.05 $11/page Learn More Microsoft is a corporation based in America that deals in developing of a vast collection of computer software and licenses. Bill Gates founded it in the year 1975 together with his friend Paul Allen. Its growth was mainly achieved from the famous windows operating systems and their tricky business licensing rights as seen in the MS-DOS. Micr osoft is also well known for its most preferred office suite in the world that is commonly used for instance the Microsoft office 2003, 2007, 2010 that are common in the computer market (Coursey, 2009, p.1). Differences Since both deal in the development of operating system a distinction is made in the release and their uses. For instance when Microsoft is producing operating systems they produce one operating system but with several versions of the same. This is seen in windows xp where we have service pack one, two and three or in windows vista home basic, business and ultimate. They produce them in such a way that it’s the same only a few features have been improved or upgraded. Contrary Apple releases are final such that only one operating system with no upgrades. They also ensure that their operating systems give their customers maximum satisfaction. Apple specializes in both hardware and software production in that it manufactures the computer itself and the operating s ystem. The Apple operating systems only function on Mac computers which are produced and distributed by Apple incorporation (Coursey, 2009, p.1). Their operating systems do not function well on other non-Apple computers. Unlike Apple, Microsoft only deals in software production and distribution. They do not produce computers and thereby the sell their software to computer manufacturers such as dell, Hewlett-Packard, Toshiba, Lenovo among others. The computer manufacturers then sell their computer brands with Microsoft operating systems installed.Advertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Microsoft operating systems are compatible with almost all computers. This implies that Microsoft operating system can run on any computer in the world today except the Mac. Unlike Microsoft that runs on many computer brands, Apple operating system and software are only meant for Mac computers only. T his means that apple products are exclusively meant for Mac computers only. Similarities Both Apple and Microsoft corporations are worldwide companies that deal in the development and distribution of computer software and operating system. The most recent Microsoft operating systems include windows seven, vista and server among others while Apple’s most recent is the Mac OS X v10.6 Snow Leopard (Bleeker, 2010, p.1). The two companies provide software that help in the running of a computers and other electronic gadgets. The two just like any other businesses have their goals and objectives. These include making profits and satisfying their customers’ wants and needs. They have to make sure they stay in business and it is through this that they have come up with a licensing program. The license helps to prevent imitation of their products and that is why genuine products from both companies have serial numbers or product keys. Conclusion In a nutshell Microsoft and Apple Corporation both deal with software and hardware development and distribution. They mainly aim at providing solutions brought about by the desires and wants of their customers. Their products have become part of our lives that we cannot do without due to their application in the daily lives today. Reference List Bleeker, E. (2010). Apple vs. Microsoft: Who Owns the Future? Retrieved from https://www.fool.com/investing/general/2010/06/04/apple-versus-microsoft-who-owns-the-future.aspx Coursey, D. (2009). Microsoft vs. Apple: Running stupid, Not Scared. Retrieved from https://www.pcworld.com/article/168603/Microsoft_vs_Apple_Running_Stupid_Not_Scared.html This essay on Microsoft vs. Apple was written and submitted by user N1k0las to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Friday, March 6, 2020

How to Avoid Bias in Your Writing

How to Avoid Bias in Your Writing How to Avoid Bias in Your Writing How to Avoid Bias in Your Writing By Mark Nichol Gender and ability bias in language doesn’t register for many people, but that’s often because many of them do not belong to the classes who have been subjected to the bias. For example, many writers persist in referring to our species, collectively, as man or mankind, even though several reasonable alternatives exist: the human race, humankind, and humanity. Most (though not all) are men. â€Å"Get over it† is a common counterargument to the assertion that because half of mankind is womankind, a gender-neutral alternative is more sensitive to that fact; man and mankind, the reasoning goes, have sufficed for most of recorded human history sorry, I mean â€Å"man history† and everybody knows it refers not just to the breadwinner, the man of the house, the king of the castle but also to the weaker sex, the little woman, the housewife. Get my drift? Get over it, indeed. Man up, and join the human race. One justification for opposing gender-neutral language is that it can be so cumbersome. Why convolutedly change he, as a generic term, to â€Å"he or she,† or his to â€Å"his or her†? We all know he or his can refer to a man or a woman, and English lacks an inclusive pronoun. (Except that it doesn’t but I’ll get to that in a moment.) Yes, repetitious use of â€Å"he or she† or â€Å"his or her† is ridiculous, but it’s easy to mix it up with it, the magical indeterminate pronoun, or to alternate between he and she or his and her in successive anecdotes, or to pluralize a reference and use they in place of a specific pronoun. Or gasp! you can replace â€Å"he or she† with they. Kill the klaxon, switch off the warning lights, and think about it: They has been long used as a singular pronoun as well as a plural one. But not everybody agrees, so be prepared for pushback if you employ this solution. References to physical disabilities are even more fraught with risks to sensitivity. Such constructions as â€Å"confined to a wheelchair† identify people by their limitations, which is discriminatory. It’s more respectful to refer to someone who â€Å"uses a wheelchair.† What about, simply, â€Å"wheelchair users,† or â€Å"blind people,† or â€Å"deaf children†? These phrases violate what’s known as the people-first philosophy, which holds that any reference to a person should emphasize the person, not their disability. So, refer to â€Å"Smith, who uses a wheelchair,† â€Å"people who are blind† or â€Å"people with visual impairments,† and â€Å"children who are deaf† or â€Å"children who are hearing impaired.† And it should go without saying that references to a disability are extraneous unless it is relevant to the discussion. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Business Writing category, check our popular posts, or choose a related post below:Comparative Forms of Adjectives15 Words for Household Rooms, and Their Synonyms48 Writing Prompts for Middle School Kids

Wednesday, February 19, 2020

Enzyme-linked immunosorbent assay Term Paper Example | Topics and Well Written Essays - 2000 words

Enzyme-linked immunosorbent assay - Term Paper Example Most of the time, this waste is hazardous and may cause detrimental effect to any living organisms, including human (Freedman, 3-9). One of the most common environmental toxicant includes the polychlorinated biphenyls or PCBs. PCBs are man-made organic chemicals belonging to a broad family of hydrocarbons. It was used in many industrial and commercial applications because of its good chemical properties like being non-flammable, high boiling point, chemical stability and good electrical insulating properties. These compounds are widely used for many applicants such as transportation and coolants. PCBs are also used as plasticizers in paints, plastics, and rubber products. It was first manufacture during 1929 but their use had been banned in 1979 upon determining its dangerous effects to both the environment and human health (Davis and Wade, 2-6). PCBs have a range of toxicity and vary in consistency from thin, light-colored liquids to yellow or black waxy solids. These toxic compound s are considered to be persistent organic pollutant for it do not degrade easily and may remain for a long time in the environment. PCBs can contaminate some parts of plants and food crops. PCBs may also be taken up into the bodies of small organisms in that lake including fishes. These toxic compounds may bioaccumulate in these food sources and ingestion may have many adverse health effects in human with symptoms such as skin condition or changes in the blood which may lead to liver damage. Some food which contains PCBs can cause poisoning to the consumers. Moreover, some studies report the health effect of PCBs in children of mothers who were exposed to PCBs (ATSDR, 285-290). Hence, it is important to detect this compound in the environment to prevent its hazardous effect. One of these methods of detection includes the use of Enzyme-Linked Immunosorbent Assay or ELISA Analytical Technique Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique which allows rapid scree ning and quantification of an antigen in a sample. It is used mainly to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and as detection or measuring tool of environmental toxicants. In ELISA, an antigen is affixed to a surface and a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, which is most commonly a color change in a chemical substrate (Lequin, 2415-2416). Enzyme-linked immunosorbent assay or (ELISA) analysis biochemical technique is used to measurement of organic contaminants such as polychlorinated biphenyls in an environmental sample. Performing an ELISA includes at least one or more antibody with specificity for an antigen. The sample, which has unknown amount of antigen, is immobilized on a solid support either non-specifically or spec ifically. The immobilization process occurs in two ways. One process is by the adsorption to the surface wherein a sample containing an antigen is adsorbed into an inert surface, usually a 96-well polystyrene well. Another process involves capturing another antibody specific to the same antigen, as in a "sandwich" ELISA (Lequin, 2417). The detection antibody is added after the antigen is immobilized, forming a complex with the antibody. This is usually done by washing the surface with a solution of a non-specific protein to block proteins

Tuesday, February 4, 2020

INTERCULTURAL Essay Example | Topics and Well Written Essays - 500 words

INTERCULTURAL - Essay Example on the positive altitude of her friends and parents, where they did not allow the intimidation of the civil rights movement to inflict fear on their children, rice was trained to remain positive in every aspect of live (The right scoop, 2010). During her interview with Kate Couric, Rice sounds positive concerning their hard task of facing their two enemies that threatened their national security. She accepts the fact that both Iraq and North Korea were dangerous in their own way but she assured her that they were going to deal with both of them separately (Schneider, 2010). The parents to Condoleezza tried their best to protect their little gal from the areas that would bring her face to face with discrimination, but though she was young, she was so much responsive to the civil rights struggle and the concerns that pertained to Birmingham. She is also seen to be actively involved in settling the disputes that existed between U.S and North Korea together with Iran. As witnessed in her childhood experiences, it is clear that Rice was an intelligent child, where her intelligence is also exhibited in the manner she handles the insecurity concerns brought about by North Korea and Iran. She believes in negotiations between rivalry communities, and dialogue with your own enemies (Schneider, 2010). After being asked by Kate on whether her government has ever taken the initiative to conduct negotiations with their enemies, she says that, they have made an effort to engage North Korea in the framework of six party talks. Having grew in a terrorism atmosphere, Rice in her interview with Kate is seen to revolve around the aspect of maintaining peace with his rivalry communities, where she is even going to the extent of involving the neighbouring communities to assist the US in convincing the North Korea and Iran to abandon their intentions of embarking on using weapons of mass destruction (The right scoop, 2010). Having seen what rivalry could bring during her early days of